Rat intestinal dendritic cells: immunostimulatory potency and phenotypic characterization.
نویسندگان
چکیده
Dendritic cells (DC) acquire antigens in peripheral tissues, transport them to lymph nodes and present peptides to T cells. DC are particularly good activators of resting T cells. Murine Langerhans' cells (LC) are efficient at endocytosing and processing antigens but are very weak immunostimulators. In culture LC lose the ability to process antigen and become potent immunostimulators. Other peripheral DC are not well characterized and it is not known if they are similarly weak immunostimulators. We isolated DC from rat Peyer's patches (PP) and lamina propria (LP) of the small intestine, from intestinal lymph (LDC) and mesenteric lymph nodes, and examined their ability to stimulate an allogeneic mixed leucocyte reaction (MLR). Freshly isolated LP DC and PP DC could stimulate a moderate MLR but fresh LDC were significantly more potent. After overnight culture, LDC did not change their potency but DC from LP and PP became as potent as LDC. In contrast, fresh lymph node DC stimulated a MLR or oxidative mitogenesis as efficiently as LDC. These results show that the weak immunostimulation of murine LC is not characteristic of all peripheral DC. We compared the phenotypes of DC from different sites before and after culture. Different populations of DC show marked phenotypic heterogeneity in the expression of surface markers, particularly Thy-1, CD2 and the iC3b receptor. PP and LP DC were similar to MLN DC in their expression of markers, but differed from LDC. After culture there were marked changes in DC surface marker expression and the differences between the populations were reduced. These observations suggest that the heterogeneity observed in fresh populations does not signify different stages of maturation but may represent activation.
منابع مشابه
In Vivo Effects of Calcitriol on Phenotypic and Functional Properties of Dendritic Cells
Background: Dendritic cells (DCs) play a central role in the initiation and expansion of T cell mediated immune responses with potential immunotherapy application. The compounds which have the ability to induce immunomodulatory effects on DCs may be employed for the treatment of immunopathologic conditions such as autoimmune diseases. Objective: The aim of this study was to investigate the in v...
متن کاملGeneration of Mature Monocyte-Derived Dendritic Cells in the Presence of Heparin and Monocyte Conditioned Medium: Phenotypic and Functional Comparison
Background: Dendritic cells (DC) induce tumor or pathogen-specific T cell responses in humans. Several laboratories have developed culture systems, including maturation factors for human DC from peripheral blood monocytes. We comprehensively compared standard maturation stimulus, an autologous monocyte-conditioned medium (MCM), with heparin for their ability to promote uniformly mature DC that ...
متن کاملCandida albicans of Vaginal Dendritic Cells in a Rat Model of Phenotypic and Functional Characterization
متن کامل
Phenotypic and Functional Comparison between Flask Adherent and Magnetic Activated Cell Sorted Monocytes Derived Dendritic Cells
Background: Generation of an effective dendritic cell (DC) based cancer vaccine depends on appropriate differentiation of monocytes in vitro. Objective: To compare the effects of monocyte separation methods, flask adherence (Flask-DC) and magnetic activated cell sorting (MACS-DC), on phenotypic and functional characteristics of resultant DCs. Methods: DCs from healthy volunteers were generated ...
متن کاملActivation-Induced TIM-4 Expression Identifies Differential Responsiveness of Intestinal CD103+ CD11b+ Dendritic Cells to a Mucosal Adjuvant
Macrophage and dendritic cell (DC) populations residing in the intestinal lamina propria (LP) are highly heterogeneous and have disparate yet collaborative roles in the promotion of adaptive immune responses towards intestinal antigen. Under steady-state conditions, macrophages are efficient at acquiring antigen but are non-migratory. In comparison, intestinal DC are inefficient at antigen upta...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Immunology
دوره 85 1 شماره
صفحات -
تاریخ انتشار 1995